TRV2-cDNA Library

80 – 96 well plates are available

We constructed a normalized cDNA library in pTRV2-GATEWAY vector (pYL279) using Poly (A) + RNA isolated from a 1:1 mixture of tissue derived from TMV resistant (transgenic Nicotiana benthamiana containing the N gene; referred to as Benth::NN) and TMV susceptible (N. benthamiana) plants 0, 1, 4 and 8 hours after TMV infection. To construct this library we first made normalized cDNA library in GATEWAY vector pCMVSport6.1. Our library had 99 fold reduction of highly expressed gene EF-1 a. Primary library contains 7.7x10 6 cfu. Average size of the insert is about 1.42 kb. This cDNA library was swapped into pTRV2-GATEWAY vector. We transformed this library into Agrobacterium strain GV2260 and hand picked colonies to generate 80 – 96 well plates.