Protocols

Detailed description of methods used to perform VIGS are described in the following articles:

*Burch-Smith, T.M., *Schiff, M., *Liu, Y., (*contributed equally) and Dinesh-Kumar, S.P. (2006) Efficient virus induced gene silencing in Arabidopsis thaliana. Plant Physiology. 142:21-27. [abstract]

*Burch-Smith TM, *Anderson JC, (*contributed equally), Martin GB, Dinesh-Kumar SP. (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J. 39:734-746. [abstract]

Dinesh-Kumar SP, Anandalakshmi R, Marathe R, Schiff M, Liu Y. (2003) Virus-induced gene silencing. Methods Mol Biol. 236:287-294. [abstract]

Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP. (2002) Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J. 30:415-429. [abstract]

Liu Y, Schiff M, Dinesh-Kumar SP. (2002) Virus-induced gene silencing in tomato. Plant J. 31:777-786. [abstract]

Ekengren SK, Liu Y, Schiff M, Dinesh-Kumar SP, Martin GB. (2003) Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato. Plant J. 36:905-917. [abstract]